Upstream stimulatory factors stimulate transcription through the E-box motifs in the PF4 gene in megakaryocytes Short title: USF1 and 2 regulate PF4 gene expression in megakaryocytes

نویسندگان

  • Kazuta Yasui
  • Robert W Jackman
  • Toru Nakano
  • Takefumi Doi
  • Yoshiaki Okada
  • Eri Matsuura
  • Zenzaburo Tozuka
  • Ryohei Nagai
  • Ayako Watanabe
  • Kayoko Matsumoto
  • Robert W. Jackman
چکیده

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Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes.

Platelet factor 4 (PF4) is expressed during megakaryocytic differentiation. We previously demonstrated that the homeodomain proteins (myeloid ecotropic integration site 1 [MEIS1], Pbx-regulating protein 1 [PREP1], and pre-B-cell leukemia transcription factors [PBXs]) bind to the novel regulatory element tandem repeat of MEIS1 binding element [TME] and transactivate the rat PF4 promoter. In the ...

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Upstream stimulatory factors stimulate transcription through the E-box motifs in the PF4 gene in megakaryocytes

Yoshiaki Okada, Eri Matsuura, Zenzaburo Tozuka, Ryohei Nagai, Ayako Watanabe, Kayoko Matsumoto, Kazuta Yasui, Robert W. Jackman, Toru Nakano and Takefumi Doi* Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan Biopharmaceutical and Pharmacokinetics Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., 2-1-6 Yodogawa-ku, Osaka 532-8514, ...

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Multiple ETS Family Proteins Regulate PF4 Gene Expression by Binding to the Same ETS Binding Site

In previous studies on the mechanism underlying megakaryocyte-specific gene expression, several ETS motifs were found in each megakaryocyte-specific gene promoter. Although these studies suggested that several ETS family proteins regulate megakaryocyte-specific gene expression, only a few ETS family proteins have been identified. Platelet factor 4 (PF4) is a megakaryocyte-specific gene and its ...

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The transcription factor GATA1 coordinates timely activation and repression of megakaryocyte gene expression. Loss of GATA1 function results in excessive megakaryocyte proliferation and disordered terminal platelet maturation, leading to thrombocytopenia and leukemia in patients. The mechanisms by which GATA1 does this are unclear. We have used in vivo biotinylated GATA1 to isolate megakaryocyt...

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Characterization of megakaryocyte GATA1-interacting proteins: the corepressor ETO2 and GATA1 interact to regulate terminal megakaryocyte maturation.

The transcription factor GATA1 coordinates timely activation and repression of megakaryocyte gene expression. Loss of GATA1 function results in excessive megakaryocyte proliferation and disordered terminal platelet maturation, leading to thrombocytopenia and leukemia in patients. The mechanisms by which GATA1 does this are unclear. We have used in vivo biotinylated GATA1 to isolate megakaryocyt...

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تاریخ انتشار 2004